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Pdgfrb-creer tdtomato

Splet10. nov. 2024 · Material and methods The NG2-CreERTM-tdTomato and the PDGFRb-P2A-CreERT2-tdTomato mice were treated with tamoxifen at three defining time points of retinal vascular development: postnatal days (P ... SpletDownload scientific diagram Generation of a pericyte-specificc Cre line. a, Constructs: Pdgfrb promoter expressing Flp and Cspg4 promoter driving the Frt-Stop-Frt-CreER cassette. Crossing ...

A suite of new Dre recombinase drivers markedly expands the …

Splet02. feb. 2024 · First, Pdgfrb-CreER;R26-tdTomato mice were treated with tamoxifen at P14, and hearts were harvested for targeting analyses at P21 (Figure 4 A). Immunostaining for … Splet24. jun. 2024 · The Pdgfrb-Flp; Cspg4-FSF-CreER line was characterized further by crossing to Ai14 Cre reporter mice that harbor a loxP-flanked STOP cassette that prevents … instagram story maker online https://comperiogroup.com

Generation of a pericyte-specificc Cre line. a, Constructs: Pdgfrb ...

Splet30. maj 2024 · Fgd5-CreER; tdTomato mice were injected with tamoxifen for 5 days, then with 5-FU at 2 weeks after the first tamoxifen injection. Peripheral blood (PB) was analyzed at weeks 3 and 4 (n = 3-4). (F) Peripheral blood analysis for tdTomato + cells in the indicated population revealed increased tdTomato + cells after 5-FU treatment. All data ... SpletThe NG2-CreER™-tdTomato and the PDGFRb-P2A-CreER T2-tdTomato mice were treated with tamoxifen at three defining time points of retinal vascular development: post-natal … SpletTandem-dimer tomato (tdTomato) is a stable, robust red fluorescent protein that is nearly threefold brighter than GFP. Our objective was to create a line of transgenic rats that ubiquitously express tdTomato in all cells, driven by the human ubiquitin C promoter. We sought to determine the rats' utility in tissue engineering applications by ... instagram story maximum time

A suite of new Dre recombinase drivers markedly expands the …

Category:Multiple cell types contribute to the atherosclerotic lesion ... - Nature

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Pdgfrb-creer tdtomato

Contribution of PDGFRα-positive cells in maintenance and injury ...

Splet25. nov. 2024 · dTomato来源于一种被命名为dimer2的中间体，这种中间体是在四聚体DsRed蛋白崩解过程中产生的。 这种变体包含GFP的N末端和C末端的前后各七个氨基酸残基，这样可以提高与融合蛋白的兼容性并降低 … Splet07. mar. 2024 · The PDGFRα-CreER transgenic mouse was first developed to study oligodendrocyte precursor cells in the forebrain and spinal cord of adult mice. 27 The …

Pdgfrb-creer tdtomato

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To study the adipogenic potential of PDGFRb + cells in adult hearts, we administered Pdgfrb-CreER;R26-tdTomato mice with tamoxifen at P8W and collected the hearts at P18W (Figure S3A). Immunostaining results showed that both intramyocardial and subepicardial adipocytes were partially labeled by tdTomato ( Figures S3 B and S3C), suggesting that ... Splet06. apr. 2024 · Specifically, the PDGFRβ promoter drives expression of Flp recombinase, which removes the Frt-flanked stop sequence, enabling expression of CreER under the …

Splet030201 B6.Cg- Pdgfrb tm1.1(cre/ERT2)Csln /J PDGFRβ-P2A-CreER T2 mice express tamoxifen-inducible Cre recombinase under the direction of the murine platelet derived …

Splet本发明提供一种Cx3cr1CreER‑Ai27基因型双转鼠以及一种新的小胶质细胞激活方法，通过Cx3cr1CreER小鼠与Ai27基因小鼠进行杂交获得Cx3cr1CreER‑Ai27基因型双转鼠，在腹腔注射Tamoxifen后于目标脑区植入光纤，使用蓝光(450‑490nm)刺激，即可特异性激活光纤下方区域小胶质细胞。 Splet21. apr. 2024 · We then treated Pdgfra-CreER; R26 tdTomato mice with tamoxifen and harvested the carotid artery and ascending aorta on day 10 to examine the initial labeling …

Splet南模生物为该研究构建了多种基因修饰小鼠： R26-LSL-RSR-tdT-DTR, Tnnt2-Dre-Cre-tdTomato, Npr3-tTA以及Col1a2-RSR-CreER等。 之前的研究主要用单同源重组酶介导的谱系示踪方法，通过心内膜的遗传工具小鼠和心外膜的遗传工具小鼠去研究心内膜衍生的成纤维细胞和心外膜衍生 ...

Splet01. feb. 2024 · The protein encoded by the PDGFRB gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family [27]. PDGFRB plays an … jewelry manufacturing softwareSplet21. apr. 2024 · We then treated Pdgfra-CreER; R26 tdTomato mice with tamoxifen and harvested the carotid artery and ascending aorta on day 10 to examine the initial labeling (Figs. 1 C–E). While labeled cells... jewelry manufacturing inventory softwareSpletCrossing: Pdgfrb-Flp;Cspg4-FSF-CreER mice × Ai14 tdTomato line. TAM, tamoxifen. b–f, Characterization of the pericyte-specific Cre line with the Ai14 reporter mice. b, Expression of... jewelry manufacturers chinaSplet07. maj 2024 · To examine adult PDGFRα + and PDGFRβ + cell labeling with an inducible system, we used Ubc-CreER tg to globally express tamoxifen (Tmx)-regulated Cre under control of the ubiquitin C promoter. As there is no perfect Cre driver for APs, we chose Ubc-CreER tg to be as inclusive as possible in our analysis, relying on the endogenous Pdgfra … jewelry mark crown with p dSplet31. maj 2024 · E, immunostaining for tdTomato, CDH5, and PDGFRb on heart sections from adult Nfatc1–Dre;Sox9–CreER;Ai66. The arrows indicate tdTomato + PDGFRb + pericytes. The boxed regions in the left panel are magnified and split channels in the right panels. Scale bars, 100 μm. jewelry mark diamond shapeSplet27. jul. 2024 · Six-week-old PDGFRβ-P2A-CreER T2; Rosa-tdTomato mice were treated with tamoxifen intraperitoneally for five consecutive days, and the retinal vasculature was … jewelry manufacturers indiaSplet14. apr. 2024 · Expression profiling by high throughput sequencing. Summary. Various transgenic CreER;tdTomato mouse-lines (Pdgfrb-CreER, Col1a1-CreER, Gli1-CreER, Cdh5 … jewelry marked 925 italy