Cstps1

WebNon-small cell lung carcinoma (NSCLC) cells with oncogenic KRAS and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains … WebSep 1, 2011 · Expression of a cytosolic form of CsTPS1 in addition to mtCsTPS1 further elevated valencene production levels by approximately 25%, an amount (300 μg/l) that …

Deficiency of valencene in mandarin hybrids is associated …

WebMay 18, 2016 · Valencene synthesis is catalyzed by the terpene synthase CsTPS1, but the transcriptional mechanisms controlling its gene expression are unknown. Here, the AP2/ERF (APETALA2/ethylene response factor) transcription factor, CitAP2.10, is characterized as a regulator of (+)-valencene synthesis. WebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase-deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 (this … orchideen ohne topf https://comperiogroup.com

Production of the sesquiterpene (+)-valencene by metabolically ...

WebOther disorders. One common alteration (polymorphism) in the CPS1 gene has been associated with increased risk of circulatory problems in newborns and in individuals who … WebNov 5, 2024 · Introduction: The ability to reprogram cellular metabolism including enhanced de novo nucleotide synthesis is a hallmark of cancer and essential for tumor progression and survival. The pyrimidine … WebThe recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass … orchideen mallorca

Characterization of˜a˜monoterpene synthase gene from

Category:Citrus fruit flavor and aroma biosynthesis: isolation

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Cstps1

Citrus fruit flavor and aroma biosynthesis: isolation, …

WebTwo recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. … CsTPS1 has previously been characterized as the key gene encoding the enzyme terpene synthase that catalyzes (+)-valencene biosynthesis (Sharon-Asa et al., 2003). Therefore, we screened a large set of citrus transcription factors for activation or inhibition using the promoter of CsTPS1 and a dual-luciferase assay ( Supplementary Fig. S1 ).

Cstps1

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Web446 Planta(2024)247:443–457 13 furtherpuriedandsequenced.Theresultingsequencewas named CsTPS1 pro. Thereafter,theCsTPS1 pro sequencewasfusedtoa GUSreportergene.Forthis,368nucleotidesupstreamthe WebJun 1, 2004 · The characterization of the Cstps1 gene now provides researchers with an opportunity to engineer plants for high-level production of valencene, a highly valued flavor and fragrance compound. Adapted …

WebMar 1, 2007 · Recombinant CsTPS1 showed a K m value of 6.8 μM, a V max of 1.1 × 10 −4 μmol/min and V max /K m of 0.016; the pH optimum was determined at pH 6.5, and a temperature optimum at 40°C. Recombinant CsTPS2 showed a K m value of 10.5 μM, ... WebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase‐deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 (this work), Nicotiana tabacum epi‐aristolochene synthase (Accession # T03714), and Gossypium arboreum cadinene synthase (Accession # CAA77191).Conserved sequence …

WebCsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa. The work describes the in vitro characterization of a monoterpene synthase and its regulatory region that we cloned from Camelina sativa (Camelina). Here, we named this gene as C. sativa terpene synthase 1 … WebJun 1, 2004 · The corresponding residues within Cstps1 were initially identified by primary sequence alignment, then visual inspection of the relevant sequences overlaid onto the …

WebCstps1 belonged to the terpene synthase-a subfamily of plant, which was the largest clade of terpene synthases and mainly composed of sesquiterpene synthases. The Cstps1 expression was detected in different organs, with high expression levels in leaf and flower, weak expression in stem and root. Real-time qRT-PCR indicated that Cstps1

WebOct 19, 2024 · CsTPS1-3 have predicted chloroplast-target peptides and produced predominantly linalool when GPP was supplied as substrate. Furthermore, CsTPS9 catalysed the formation of ( E )-β-ocimene and myrcene, and small amounts of sabinene and ( Z )-β-ocimene from GPP. ir526 form 2022 print offWebBiochemical goals • In Citrus sinensis, the Cstps-1 gene codes for the production of the enzyme Citrus Valancene Synthase (CVS). • In vivo, CVS reacts with farnesyl pyrophosphate (FPP) to produce the characteristically fragrant valencene (as well as 5 … ir56 taxpayer registrationWebDec 1, 2003 · Cstps1 was found to be developmentally regulated: transcript was found to accumulate only towards fruit maturation, corresponding well with the timing of valencene accumulation in fruit. Although citrus fruits are non‐climacteric, valencene accumulation and Cstps1 expression were found to be responsive to ethylene, providing further evidence ... ir56g form downloadWebTwo recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. ‘Skunk’ trichome mRNA, have been isolated and characterized. Recombinant CsTPS1 showed a Km value of 6.8 μM, a Vmax of 1.1 x 10-4 µmol/min and V ir56 form preparation toolWebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. ir56e form downloadWebSep 30, 2024 · Nimbus Therapeutics Publishes Structural Analysis Highlighting Mechanisms for Selective Inhibition of CTPS1. CAMBRIDGE, Mass. – September 30, 2024 – Nimbus … orchideenclipseWebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. ir56m english