Cisplatin hela

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WebIn vitro response to ionizing radiation (IR), cisplatin (cDDP), hyperthermia (HT) and PARP1-inhibition (PARP1- i) as single and combinational treatments in four cervical cancer cell lines (SiHa, HeLa, CaSki and C33A). ( A) Normalized colony formation after IR in combination with cDDP or ( B) with HT. WebAbstract. To study the apoptosis induced by cisplatin in cervical cancer cell line HeLa and its mechanism, cell growth inhibition of cisplatin on HeLa cells was analyzed by …

(PDF) CRISPR/Cas9-mediated knockout of MLL5 enhances …

WebFor IFNβ-cisplatin sequential treatment protocol (E), HeLa cells were treated with 100 ng/mL IFNβ for 24, 48, or 72 h, and then cells were replated in 96-well plates and treated … WebMay 15, 2008 · Summary. To study the apoptosis induced by cisplatin in cervical cancer cell line HeLa and its mechanism, cell growth inhibition of cisplatin on HeLa cells … cincinnati to oxford ohio

MBNL1 regulates resistance of HeLa cells to cisplatin via Nrf2

WebNational Center for Biotechnology Information WebIn order to assist in optimizing treatment, we applied 5-ALA/PDT in combination with low-dose cisplatin to evaluate cytotoxicity in Hela cells. Methods: Antiproliferative effects of 5-ALA/PDT and cisplatin, alone and in combination, were assessed using MTT assay. Web1 day ago · To further confirm the autophagic effect, the expression level of LC3 was detected by immunoblot analysis in HeLa cells, an appropriate tool cells for ... Pomiferin potentiates the tumor suppression effect of cisplatin via attenuating ABCB1 activity and promoting cisplatin accumulation in tumor tissues of RM-1 drug-resistant prostate ... dhu home visiting service

CaSki, SiHa, HeLa, and C33A cervical cancer cells display …

Nucleolin Promotes Cisplatin Resistance in Cervical Cancer by the …

WebAug 6, 2024 · Cisplatin is a platinum-based chemotherapeutic agent that is widely used for the treatment of malignant tumors, such as cervical cancer, lung cancer, and ovarian cancer [3]. Cisplatin mainly induces cross-linking at the N7-position of guanosine, which modifies DNA to induce apoptosis and kill cancer cells [4]. WebJun 1, 2024 · IC90 of paclitaxel in HeLa Pac RB was increased from 69 pM, 440 pM, 2,561 pM and 10,337 pM on 0th, 1st, 2nd, 3rd and 4th months, respectively. Interestingly, the resistant cells were recovered to... cincinnati to parkersburg west virginiaWebThe cis-Diammineplatinum (II) dichloride (cisplatin; P4394), 3- (4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT; M2128), and dimethyl sulfoxide (DMSO; D2650 for cell stock storage and D5979 for drug preparation) were purchased from Sigma (St. Louis, MO, USA). cincinnati top employers

"WebResults: HeLa and SiHa cells were more sensitive to cisplatin and oxaliplatin treatment than C33A cells. HeLa and SiHa cells had significantly lower NRF2 gene levels, NQO1 … " - Cisplatin hela

Cisplatin hela

Roles of SLX1–SLX4, MUS81–EME1, and GEN1 in avoiding genome …

WebAug 21, 2024 · Effect of cisplatin on the proliferative activity and survival of cancer cells. ( A) Viable cell number for HeLa and HeLa-SypHer2 cells. ( B) Percentage of dead (trypan blue positive) cells.... WebJan 20, 2024 · Further investigation revealed that cisplatin-resistant cells sustain OXPHOS partly via iron metabolism reprogramming during glutamine deprivation. This reprogramming might be responsible for mitochondrial iron-sulfur [Fe-S] cluster biogenesis, which has become an "Achilles' heel," rendering cancer cells vulnerable to DFO-induced autophagic ...

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WebCisplatin (DDP) is one of the major chemotherapeutic drugs for cervical cancer patients. But, drug resistance limits the effectiveness of cancer therapy. Nucleolin (NCL) is a … WebDrug: Cisplatin - Cancerrxgene - Genomics of Drug Sensitivity in Cancer Genomics of Drug Sensitivity in Cancer Compound: Cisplatin Drug Target: DNA crosslinker Drug Target …

WebFeb 16, 2024 · severe or ongoing nausea and vomiting; vision problems, pain behind your eyes; hearing problems, ringing in your ears; pain, redness, swelling, or skin changes … WebApr 11, 2024 · HIGHLIGHTS who: Umit Aliyaskarova from the Universitu00e9 Nazarbayev University have published the paper: NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells, in the Journal: Scientific … Neil3-mediated proteasomal degradation facilitates the repair of cisplatin-induced dna …

WebFeb 10, 2024 · Cisplatin is one of the most effective chemotherapy drugs and is widely used in the treatment of cancer, including hepatocellular carcinoma (HCC) and … WebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often …

WebFeb 28, 2024 · One of the main problems in chemotherapy using platinum drugs as anticancer agents is the resistance phenomenon. Synthesizing and evaluating valid alternative compounds is challenging. This review focuses on the last two years of progress in the studies of platinum (II)- and platinum (IV)-based anticancer complexes. In …

WebMixed lineage leukemia 5 (MLL5) transactivates the expression of E6 and E7 oncogenes in cervical cancer cells. In this study, we utilized CRISPR/Cas9 system with the aim to … cincinnati to petersburg kyWebCisplatin, as a significant chemotherapeutic drug for the treatment of cancers, was combined with rapamycin (RAPA), an autophagy inducer, or 3-methyladenine (3-MA), an autophagy inhibitor, and these cisplatin … dhu healthcare reviewsWebJan 12, 2024 · Cisplatin-resistant HeLa cells were transfected with NHERF1 and overexpression of NHERF1 protein was confirmed by Western blotting ( Figure 2 A). Effect of NHERF1 on cell growth was examined by CCK8 … dhu home visiting service leicesterWebApr 21, 2024 · Cisplatin, as a significant chemotherapeutic drug for the treatment of cancers, was combined with rapamycin (RAPA), an autophagy inducer, or 3 … dhu heartach lighthouseWebSep 10, 2010 · Cisplatin has been widely accepted as one of the most efficient anticancer drugs for decades. However, the mechanisms for the cytotoxic effects of cisplatin are still not fully understood. Cisplatin primarily targets DNA, resulting in the formation of DNA double strand breaks and eventually causing cell death. dhu homöopathie campusWebJul 1, 2009 · To test our hypothesis, we employed HeLa Tet (tetracycline)-off cells, which we previously used to overexpress the native plaa gene [21], and determined their apoptotic responses to cisplatin. HeLa cells were chosen because cervical tumor is a cisplatin-responsive tumor, while cervical tissues express the plaa gene [31], and respond to ... dhu healthcare logoWebFeb 12, 2024 · MBNL1 regulates resistance of HeLa cells to cisplatin via Nrf2 Chemotherapy is an important method in the treatment of cervical cancer, but some patients will face drug resistance, which often indicates a poor prognosis. Moreover, there is no complete solution at present. cincinnati to philadelphia flights